Polymerase chain reaction, or PCR, is a laboratory technique used to make multiple copies of a segment of DNA. PCR is very precise and can be used to amplify, or copy, a specific DNA target from a ...

When the first thermal cyclers for the polymerase chain reaction came out in the 1980s, they were as expensive as a market driven by grant money could make them. Things haven’t got much better ...

1| Combine the following components of the master mix on ice and mix thoroughly: 10× KLA reaction buffer (pH 9.2): 500 mM Tris base, 160 mM (NH 4) 2 SO 4, 25 mM MgCl 2, 1% Tween 20. Note ...

Forensic DNA profiling uses polymerase chain reaction (PCR) to amplify short tandem repeats (STRs), which are highly variable DNA regions, typically with four repeating bases. This variability helps ...

Since the emergence of COVID-19 in late 2019, the gold standard in testing for the disease has been a nuclear-derived technique: real-time reverse transcription–polymerase chain reaction, or real-time ...