Janet Mertz’s work with simian virus 40 DNA during her graduate years was pivotal for developing DNA recombination as we now know it. In September 1970, Janet Mertz joined the biochemistry department ...

Adenoviral vectors will transduce a wide range of target cells resulting in high-level gene expression. They are non-integrating vectors so expression in dividing cells is progressively lost. First ...

Figure 1: GFP-fragment reassembly vectors and cloning linker sequences. To prepare the cloning vectors, transform separately pET11a-link-NGFP and pMRBAD-link-CGFP into an E. coli strain that gives ...

Figure 1: Retroviral backbone of pSHAG-MAGIC2. To generate the hairpin primer, select a 'sense' sequence(s) of 22 nucleotides (nt) in length from the coding sequence of the gene of interest for ...